產(chǎn)品描述
四環(huán)素陰性胎牛血清(Tetracycline Negative Fetal Bovine Serum)胎牛血清一般包含抗生素四環(huán)素(tet),當(dāng)使用非常敏感的tet-誘導(dǎo)的基因表達(dá)系統(tǒng)時,研究人員會面臨意料之外的表達(dá)問題。血清含有zui低級別的四環(huán)素是必要的,以確保精確的基因表達(dá)。胎牛血清會含有四環(huán)素,科研工作者在使用敏感四環(huán)素誘導(dǎo)調(diào)控表達(dá)系統(tǒng)時,往往會遇到非預(yù)期基因表達(dá)的問題。為確保精確的基因表達(dá),血清中所含的四環(huán)素越少越好。這款產(chǎn)品可應(yīng)用于使用敏感四環(huán)素誘導(dǎo)表達(dá)系統(tǒng)的細(xì)胞培養(yǎng),以及其它對四環(huán)素敏感的實(shí)驗中。四環(huán)素檢測含量約為0.001µg/ml。
產(chǎn)品特點(diǎn)
Quality Profile | Specification | Result | Unit |
Appearance | Clear amber | Pass | |
PH | 6.8-8.2 | 7.30 | |
Osmolality | 280-340 | 315 | mOsm/kg |
Endotoxin | As reported | 1.01 | ng/ml |
Hemoglobin | ≤25 | 14.9 | mg/ml |
Tetracycline/-derivates | ≤0.5 | Pass | μg/ml |
Glucose | As reported | 3.87 | mM |
Calcium | As reported | 2.65 | mM |
Phosphorous | As reported | 2.79 | mM |
Got/AST | As reported | 40 | U/l |
LDH | As reported | 599 | U/l |
Protein Profile | |||
Total Protein | 3.0-4.5 | 3.7 | g/dl |
Albumin | As reported | 2.2 | g/dl |
lgG | ≤250.0 | 189.00 | mg/l |
Sterility Testing | |||
Bactria and Fungi | Not detected | Not detected | |
Mycoplasma | Not detected | Not detected | |
Virus Testing | |||
Bovine Herpes Virus(BHV-1 and BHV-5) | Not detected | Not detected | |
Pestivirus(e.g.BVD-V) | Not detected | Not detected | |
Parainfluenza Virus Type 3(PI-3) | Not detected | Not detected | |
Biological Performance | |||
Cell Growth MDCK(Madin-Darby Canine Kidney Line) | ≥75.0 | 101.0 | |
%Cell Growth CHO(Chinese Hamster Ovary Cell Line) | ≥75.0 | 100.0 | % |
產(chǎn)品應(yīng)用
四環(huán)素陰性胎牛血清四環(huán)素陰性胎牛血清常用于科研、診斷試劑、疫苗生產(chǎn)和研發(fā),同時也能夠用于作為免疫反應(yīng)中的阻斷劑和蛋白配體反應(yīng)中的固定劑。
運(yùn)輸與保存方法
干冰運(yùn)輸,-20℃保存,有效期5年。
注意事項注意事項
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本產(chǎn)品主要用于科研領(lǐng)域,不宜用于臨床診斷或其他用途。
貨號 | 品名 | 規(guī)格 | 品牌 |
78ED10009-500ml | 四環(huán)素陰性胎牛血清 | 500ml | BIOHUB |
78ED10009-10*50 ml | 四環(huán)素陰性胎牛血清 | 10*50 ml | BIOHUB |
78ED10009-50ml | 四環(huán)素陰性胎牛血清 | 50ml | BIOHUB |